Culture of mouse cartilage cells on DNA films

作者：Tanabe Fuyuko^*; Suzuki Yukari; Watanuki Amane; Kuroda Masatoshi; Takahashi Youichi; Ogata Naoshi; Ogata Naoya

来源：Kobunshi Ronbunshu, 2012, 69(1).

DOI：10.1295/koron.69.33

• Cell density
• Culture dishes
• Culture plates
• Cured films
• DNA films
• Mouse cartilage
• UV cured

Pure deoxyribonucleic acid (DMA) whicn was obtained from Salmon roe was dissolved in water, followed by evaporating water to obtain clear and mechanically strong films. Since DNA is proved to be biocompatible as biomaterials, water-insoluble DNA films were prepared to apply to culture plates for mouse cartilage cells. DNA films were irradiated by UV lamp to obtain water-insoluble cross-linked DNA films which were used for cell culture. It was found that the UV cured DNA films were suitable for mouse cartilage cell culture and cells can grow up to higher cell density on UV cured DNA films than normal cell culture dishes.

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