Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model

作者：Yang, Xuesong^*; Luo, Guangyun; Fu, Lan; Huang, Hong; Wang, Lifen; Yin, Lihua; Zhang, Xuelian; Wang, Tingting; Ma, Xuan; Feng, Tianyu; Ye, Jianzhou^*

来源：Evidence-based Complementary and Alternative Medicine, 2022, 2022: 3193572.

DOI：10.1155/2022/3193572

• TRADITIONAL CHINESE MEDICINE
• CLINICAL-FEATURES
• PROLIFERATION
• KERATINOCYTES
• PHARMACOLOGY
• DISEASE

Background. Psoriasis is a common chronic inflammatory skin disease with multifactor etiology, characterized by abnormal proliferation and differentiation of keratinocytes. Huang-Lian Jie-Du decoction (HLJDD) is a traditional Chinese medicine prescription with good clinical curative effect on psoriasis. However, its therapeutic mechanisms are still unclear. Methods. The psoriasis model of SKH-1 nude mice was established by imiquimod-induced and HLJDD gavage was given. Hematoxylin and eosin staining were used to evaluate pathological morphologies, and immunohistochemistry was used to detect the expressions of Wnt1, beta-catenin, and c-Myc in psoriasis mice. Western blot was used to examine the expressions of Frizzled-2, LRP5/6, GSK-3 beta, APC, Axin2, TCF4, LEF1, cyclin D1, TBX3, EPHB2, and NOTUM enzyme. Results. In this study, HLJDD reduced skin erythema and lesions, decreased the thickness of epidermal and downregulated the expressions of Wnt1, beta-catenin, and c-Myc. Western blot results showed that HLJDD reduced the expressions of Wnt receptors Frizzled-2 and LRP5/6, and Wnt downstream target genes TCF4, LEF1, cyclin D1, TBX3, and EPHB2, while upregulated destruction complex proteins GSK-3 beta, APC, and Axin2. Conclusions. HLJDD can effectively treat psoriasis and inhibit the Wnt/beta-catenin signaling pathway at multiple stages.

• 单位
  1; 中国医科大学