DNA origami based assembly of gold nanoparticle dimers for SERS detection

Authors：Thacker Vivek V; Herrmann Lars O; Sigle Daniel O; Zhang Tao; Liedl Tim; Baumberg Jeremy J; Keyser Ulrich F

Source：Plasmonics in Biology and Medicine XII, 2015-02-07 To 2015-02-08.

DOI：10.1117/12.2076799

• Dark-field scatterings
• Dna origamis
• Far-field scattering
• Metallic nanostructure
• Nanoplasmonics
• Single-molecule analysis
• Single-stranded DNA
• Surface enhanced Raman Scattering (SERS)

Plasmonic sensors are extremely promising candidates for label-free single molecule analysis but require exquisite control over the physical arrangement of metallic nanostructures. We employ self-assembly based on the DNA origami technique for accurate positioning of individual 40 nm gold nanoparticles with gaps of 3.3 ± 1 nm. This is probed through far field scattering measurements on individual dimers. This plasmonic coupling allows us to use surface enhanced Raman scattering (SERS) to detect a small number of dye molecules as well as short single-stranded DNA oligonucleotides in the vicinity of the dimers. This demonstrates that DNA origami is a powerful tool with great potential for a wide variety of biosensing and single-molecule applications.

• Institution
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