Fluorescence Probe for Imaging N-Methyl-D-aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy

作者：Kwon, Nahyun; Lim, Chang Su; Ko, Gyeongju; Ha, Jeongsun; Lee, Dayoung; Yin, Jun^*; Kim, Hwan Myung^*; Yoon, Juyoung^*

来源：Analytical Chemistry, 2021, 93(33): 11612-11616.

DOI：10.1021/acs.analchem.1c02350

• GLUTATHIONE GSH
• RECENT PROGRESS
• CYSTEINE
• CELLS
• NAPHTHALIMIDE
• LUMINESCENT
• ANTIOXIDANT
• SITES

N-Methyl-D-aspartate (NMDA) is an excitotoxic amino acid used to identify a specific subset of glutamate receptors. The activity of NMDA receptors is closely related to the redox level of the biological system. Glutathione (GSH) as an antioxidant plays a key role with regard to modulation of the redox environment. In this work we designed and developed a GSH-specific fluorescent probe with the capability of targeting NMDA receptors, which was composed of a two-photon naphthalimide fluorophore, a GSHreactive group sulfonamide, and an ifenprodil targeting group for the NMDA receptor. This probe exhibited high selectivity toward GSH in comparison to other similar amino acids. Two-photon fluorescence microscopy allowed this probe to successfully monitor GSH in neuronal cells and hippocampal tissues with an excitation at 750 nm. It could serve as a potential practical imaging tool to explore the function of GSH and related biological processes in the brain.