Sustained Release of Collagen-affinity SDF-1alpha from Book-shaped Acellular Fibrocartilage Scaffold Enhanced Bone-tendon Healing in a Rabbit Model.

摘要

Rapid and functional bone-tendon (B-T) healing remains a difficulty in clinical practice. Tissue engineering has emerged as a promising strategy to address this problem. However, the majority of tissue engineering scaffolds are loaded with stem cells to enhance the regenerability in B-T healing, which is complicated and inconvenient for clinical application. Accordingly, developing a cell-free scaffold with chemotactic function and chondrogenic inducibility may be an effective approach. In this study, a collagenaffinity peptide(CAP) derived from the A3 domain of von Willebrand factor (a hemostasis factor) was fused into the C-terminal of a stromal cell-derived factor-1alpha (SDF-1alpha) to synthesize a recombinant SDF-1alpha capable of binding collagen and chemotactic activity. The recombinant SDF-1alpha was then tethered on the collagen fibers of a book-shaped acellularfibrocartilage scaffold(BAFS), thus fabricating a novel scaffold (C-SDF-1alpha/BAFS) with chemotactic function and chondrogenic inducibility. In-vitro tests determined that this scaffold was noncytotoxic and biomimetic, could attract stem cells migrating to the scaffold using sustainably released C-SDF-1alpha, and inducedthe interacting stem cells down the chondrogenic lineage. In vivo, the C-SDF-1alpha/BAFS significantly enhanced the B-T healing in a rabbit partial patellectomy model, as shown by the larger cartilaginous metaplasia region, better fibrocartilage regeneration,additional bone formation, and improved biomechanical properties. Therefore, the findings of the study demonstrate that the C-SDF-1alpha/BAFS could potentially be applied for B-T healing.

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