Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18

作者：Zhou, Shiying; Zhu, Shuyu; Huang, Zhen; Chen, Jian; Li, Jiawei; Yang, Mei; Jin, Liang^*; Huo, Danqun^*; Hou, Changjun^*

来源：Chemical Communications, 2023, 59(80): 11987-11990.

DOI：10.1039/d3cc04223e

• TESTS

Simultaneous detection of multiple targets can provide important data support for clinical diagnosis and treatment. Here, we report a facile isothermal assay based on target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a (TM-RCT/Cas12a-Cas13a). Through facile one-step amplification (TM-RCT), two target DNAs are converted to RNA amplified products. The simultaneous detection of HPV16 and HPV18 is then achieved by combining two CRISPR/Cas systems. This system shows excellent sensing performance and provides a universal method for simultaneous detection. @@@ Simultaneous detection of multiple targets can provide important data support for clinical diagnosis and treatment.

• 单位
  重庆大学; 四川大学