Ratiometric fluorescence determination of chlortetracycline based on the aggregation of copper nanoclusters triggered by aluminum ion

作者：Cai, Xin; Ye, Jianpin; Duan, Ding; Li, Kang^*

来源：Mikrochimica Acta, 2022, 189(1): 28.

DOI：10.1007/s00604-021-05093-5

• Copper nanoclusters
• Al3+ determination
• Aggregation-induced emission
• Chlortetracycline
• Ratiometric fluorescence detection
• Test paper

The aggregation-induced emission (AIE) characteristic of copper nanoclusters (CuNC) was for the first time used to construct a ratiometric fluorescence probe (CuNC-Al3+) for detection of chlortetracycline (CTC). Aluminum ion (Al3+) can aggregate free CuNC and make it emit a bright and stable red fluorescence. A slight excess of Al3+ in CuNC-Al3+ solution can form a CTC-Al3+ complex to limit the conformational rotation of CTC molecule and enhance CTC fluorescence. So, the red fluorescence of CuNC-Al3+ probe and the enhanced CTC fluorescence are used as a reference signal and a response signal to detect CTC, respectively. The method developed shows a good linear relationship between the CTC concentration and the fluorescence intensity ratio (I-495/I-575) in the range 0.1-3.0 mu M, and the detection limit is 25.3 nM (S/N= 3). In addition, the fluorescent color of CuNC-Al3+ probe changes from red to yellow-green as the concentration of CTC increases. Based on this observation, a fluorescent test paper has also been fabricated.

• 单位
  广东药学院