Evaluation of the pathogenesis of non-typical strain with ?-hemolysin, Vibrio parahaemolyticus 353, isolated from Chinese seafood through comparative genome and transcriptome analysis

作者：Zha, Fei; Pang, Rui; Huang, Shixuan; Zhang, Jumei; Wang, Juan; Chen, Moutong; Xue, Liang; Ye, Qinghua; Wu, Shi; Yang, Meiyan; Gu, Qihui; Ding, Yu; Zhang, Hao^*; Wu, Qingping^*

来源：Marine Pollution Bulletin, 2023, 186: 114276.

DOI：10.1016/j.marpolbul.2022.114276

• Genome-sequencing
• Comparative genome
• Transcriptome
• Aquatic food
• Virulence factors
• Caco-2 cells

Vibrio parahaemolyticus outbreaks frequently occur, causing gastrointestinal sickness owing to the consumption of aquatic foods by various virulence factors; however, the mechanism of pathogenesis is still unknown. In this study, a non-typical strain of V. parahaemolyticus, named VP353, was isolated from shrimp in China. Its comparative genome and transcriptome after infection with Caco-2 cells were examined to illustrate the mechanisms of its pathogenesis. VP353 was a tdh-trh- strain but uncommonly manifested robust cytotoxicity towards Caco-2 cells. Compared with the standard strain RIMD2210633, VP353 harbored alpha-hemolysins (hlyA, hlyB, hlyC, and hlyD) was first reported in V. parahaemolyticus and showed high diversity in the T3SS2 gene cluster. Moreover, the expression of flagella, T2SS, quorum sensing-related genes, hlyA, hlyC were upregulated, and hlyB, hlyD were down-regulated. In summary, our results demonstrate that some novel virulence factors contribute to the pathogenesis of V. parahaemolyticus infection.

• 单位
  江南大学