Recognition of hairpin DNA from coil DNA by electrospray mass spectrometry with annealing strategy

作者：Bo Zheng Yi Quan Liua Gu Yuan a Beijing National Laboratory for Molecular Sciences Key Laboratory of Bioorganic Chemistry; Molecular Engineering of Ministry of Education Department of Chemical Biology College of Chemistry; Molecular Engineering Peking University Beijing China b Shanghai Research Institute of Chemical Industry Shanghai China

来源：Chinese Chemical Letters, 2012, (04): 500-503.

• Hairpin DNA
• Annealing
• Mass spectrometry

This research presented an annealing strategy to identify hairpin DNA from coil DNA with the same base composition but different arrangements using electrospray mass spectrometry(ESI-MS).A series of single-stranded DNA were annealed with their complementary sequences,respectively.All the five pairs of hairpin DNA and coil DNA were unambiguously distinguished by ESIMS with annealing strategy.This research offers a potential method to probe the DNA structure by comparing with mass spectral characteristics.