Effects of Stress on Osteoblast Proliferation and Differentiation Based on Endoplasmic Reticulum Stress and Wntβ-Catenin Signaling Pathway

作者：Zhong, Hanming^*; Yang, Xiaoyu; Li, Shitie; Lei, Xin

来源：Contrast Media & Molecular Imaging, 2022, 2022: 8521051.

DOI：10.1155/2022/8521051

• LRP5

In order to investigate the effect of fluid shear stress on the proliferation of osteoblasts and the regulatory role of the Wnt/beta-catenin signaling pathway in cell proliferation, a new method based on endoplasmic reticulum stress and Wnt/beta-catenin signaling pathway stress-mediated was proposed. Taking MG63 osteoblasts as the research object, they were inoculated on glass slides (G group), polished titanium sheets (P group), and sandblasted acid-base treated pure titanium sheets (S group). In addition, FSS of 0 dunes/cm(2) (static group) and 12 dunes/cm(2) (stress group) were given, respectively. Then, quantitative reverse transcription-PCR (RT-qPCR) and western blot were used to detect the mRNA and protein expressions of low-density lipoprotein receptor-related protein 5 (LRP5) and beta-catenin in MG63 cells. The results showed that the expression levels of beta-catenin mRNA and protein in cells in the stress group were significantly increased (P < 0.05), and the protein expression level of LRP5 was significantly decreased (P < 0.05). The expression level of LRP5 in group S was greatly inhibited, while the expression level of beta-catenin was significantly upregulated. Therefore, FSS can stimulate the expression of LRP5 and beta-catenin in osteoblasts. Fluid shear stress can promote osteoblast proliferation in vitro; the Wnt/beta-catenin signaling pathway is involved in regulating fluid shear stress to promote osteoblast proliferation.

• 单位
  东莞市人民医院; 南方医科大学