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Effects of collagen-based coating with chitosan and ε-polylysine on sensory, texture, and biochemical changes of refrigerated Nemipterus virgatus fillets

Huang, Yongping; Nie, Ying; Zhou, Fei; Li, Biansheng; Luo, Qiulan; Zhang, Bin; Zeng, Qinpei; Huang, Yisheng*
Science Citation Index Expanded
韩山师范学院

摘要

In order to evaluate the effects of chitosan, epsilon-polylysine, and collagen on the preservation properties of refrigerated Nemipterus virgatus, samples were tested with different treatments for 10 days, namely chitosan, epsilon-polylysine and collagen (CH + epsilon-PL + CA), chitosan and epsilon-polylysine (CH + epsilon-PL), chitosan and collagen (CH + CA), epsilon-polylysine and collagen (epsilon-PL + CA), and the uncoated sample (CK). The results demonstrated that the bio-coating exhibited better preservation effects. The CH + epsilon-PL + CA, CH + epsilon-PL, CH + CA, epsilon-PL + CA treatments could significantly inhibit bacterial growth and retard the increase of total volatile base nitrogen (TVB-N), 2-thiobarbituric acid (TBA), K-value, and total viable counts (TVC) in N. virgatus fillets. The pH of all samples decreased and reached its lowest value on day 6, then increased significantly at the end of the experiment (p < .05). Water-holding capacity (WHC) of all the groups decreased continuously throughout storage, and CK reached 66.03% on day 6, which is significantly lower than CH + epsilon-PL + CA, CH + epsilon-PL, CH + CA, and epsilon-PL + CA (p < .05). On the contrary, the sensory scores of CH + epsilon-PL + CA, CH + epsilon-PL, CH + CA, and epsilon-PL + CA were significantly higher than the control, and the score of CH + epsilon-PL + CA (p < .05) was the best among all the groups. In terms of texture, CH + PL + CA also showed less cell shrinkage and tighter muscle fiber arrangement compared to other treatments. To sum up, the CH + PL + CA bio-coating proved to be a promising method for maintaining the storage quality of N. virgatus under refrigerated storage conditions.

关键词

biological preservatives chitosan collagen Nemipterus virgatus epsilon-Polylysine