Labdane and isopimarane diterpenoids from Rosmarinus officinalis solid wastes: MS/MS spectrometric fragmentations and neuroprotective effect

摘要

The aim of this study was to purify and identify new types of secondary products from Rosmarinus officinalis solid wastes after essential oil been extracted, further study their MS/MS spectrometric fragmentations and evaluate their cytoprotective effects against H2O2-induced oxidative damage in SH-SY5Y cells. Six labdane diterpenoids, five isopimarane diterpenoids and two ferulic acid esters were obtained in which three labdane diterpenoids and four isopimarane diterpenoids were undescribed compounds. The losses of hydroxyl, tigloyl, 2-methy-butanoyl, acetyl and carbonyl groups or side chains were the main elimination modes of the new compounds in MS/MS analyses. Compounds 1, 4, 6, 10 and 12 showed the ability to protect the cells against oxidative injure whose cell viability rates were more than 65% in the concentration of 1 or 25 mu M respectively. These studies broadened the understanding of secondary products of rosemary and promoted the deep developments and utilizations of rosemary after essential oil been extracted.

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