Improvement on the Antioxidant Activity of Polysaccharides by Submerged Fermentation of Shiraia bambusicola GDMCC 60438 (Ascomycota)

作者：Zhang, Yuan; Luo, You; Tian, Xiaofei^*; Wu, Zhenqiang^*

来源：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS, 2020, 22(6): 605-614.

DOI：10.1615/IntJMedMushrooms.2020035036

• Shiraia bambusicola
• fermented mycelia
• intracellular polysaccharides
• hydroxyl radical scavenging activity
• ABTS(+) radical scavenging activity
• medicinal mushrooms and fungi

Shiraia bambusicola strain GDMCC 60438, originally isolated from eastern China, was applied for production of polysaccharides through submerged fermentation with glucose and potato extracts. Compared with a poly-saccharide content of 3.94 +/- 0.92% in natural Sh. bambusicola stroma, 9.12 +/- 0.36 g intracellular polysaccharides (SIP) could be produced in 100 g freeze-dried Sh. bambusicola mycelium after an optimized fermentation. The SIP showed an 88.65 +/- 2.26% hydroxyl radical scavenging activity (HRSA) at the concentration of 4 mg/mL. It was higher than the 31.48 +/- 3.12% HRSA of Sh. bambusicola stroma polysaccharide (SSP). In the meantime, a 5 mg/mL SIP showed an equivalent 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic) acid radical (ABTS+) scavenging activity to ascorbic acid at 0.025 mg/mL. Gel chromatography analysis showed that the SIP had distinct polysaccharide fractions compared with the SSP. Submerged fermentation showed a promising prospect on high production of Sh. bambusicola polysaccharides with improved antioxidant activities.