Genome mining of actinomycin shunt products from Kitasatospora sp. YINM00002

Authors：Zhang, Zhou-Tian-Le; Sun, Hui-Bing; Ren, Zhen; Xie, Tian-Peng; Wang, Ying-Fang; Guo, Yin; Su, Xiaoyu; Yin, Min^*; Zhou, Hao^*; Ding, Zhong-Tao

Source：2023, 13(51): 36200-36208.

DOI：10.1039/d3ra07277k

• KYNURENINE FORMAMIDASE
• STREPTOMYCES
• VINCRISTINE
• STRAIN
• PURIFICATION
• BINDING

Actinomycins are known for their anti-tumor, antibacterial and antiviral activities, and in particular for the ability of actinomycin D as a clinical drug to treat a variety of cancers. In our ongoing work to obtain novel natural products from endophytic actinomycetes derived from traditional Chinese herbs, we identified the potential to produce actinomycins in YINM00002, a Kitasatospora strain derived from Polygonatum kingianum. According to genome mining, we isolated actinomycins D and V (1 and 2) and small amounts of 4-methyl-3-hydroxyanthranilic acid (4-MHA) derivates (3 and 4) from strain fermentation broth. The presence of actinrhater A (3) and actinrhater B (4) reveals a mysterious shunt pathway in the early stages of actinomycin D biosynthesis. Our study provides a fresh perspective for further discovery and modification of novel actinomycins.

• Institution
  云南大学