Effects of dietary carbohydrate levels on growth, metabolic enzyme activities and oxidative status of hybrid snakehead (Channa maculata ? ? Channa argus ? )

作者：Xu, Tiantian; Liu, Xingwang; Huang, Wenqing; Li, Guoli; Zhang, Ye; Xu, Dan^*; Wang, Guoxia^*

来源：Aquaculture, 2023, 563: 738960.

DOI：10.1016/j.aquaculture.2022.738960

• Hybrid snakehead(Channa maculata 9?
• Channa argus d)
• Dietary carbohydrate requirement
• Growth
• Metabolic enzyme activities
• Oxidative status

An 8-week feeding trial was aimed to explore the effects of dietary carbohydrate on growth performance, metabolic enzyme activities and oxidative status of hybrid snakehead (Channa maculata 9 x Channa argus d). A total of 540 healthy hybrid snakehead with initial body weight of (39.4 +/- 0.01) g were selected and randomly divided into 18 cages, with 30 in each cage. Six isonitrogenous and isolipidic diets with different levels of digestible carbohydrate (alpha-starch) levels (DCL) (0%, 5%, 10%, 15%, 20% and 25%) were fed to hybrid snake -head. Results showed that dietary carbohydrate supplementation had significant effects on final body weight (FW), weight gain (WG), feed conversion ratio (FCR) and protein efficiency ratio (PER) of hybrid snakehead in a third order polynomial (cubic) model, with the optimal level established at 10.2%-12.0%. Effects on Condition factor (CF) of hybrid snakehead was described by a second order polynomial (quadratic) relationship. Hep-atosomatic index (HSI) and viscerosomatic index (VSI) showed a linear relationship with increasing DCL. The body protein, crude lipid and hepatic glycogen content of hybrid snakehead increased with the levels of car-bohydrate supplementation (P < 0.05). Dietary supplementation with carbohydrate significantly increased the levels of plasma cholesterol (CHOL), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low -density lipoprotein cholesterol (LDL-C) in hybrid snakehead (P < 0.05). The activities of alanine aminotrans-ferase (ALT) and aspartate aminotransferase (AST) in both plasma and liver were significantly enhanced by the highest level of carbohydrate (P < 0.05). Furthermore, the levels of superoxide dismutase (SOD) and catalase (CAT) in liver increased firstly and then decreased with the increasing of DCL (P < 0.05). The expression levels of genes encoding SOD, glutathione peroxidase (GSH-PX), proinflammatory cytokines interleukin 1 beta (IL-1 beta) and interleukin 8 (IL-8) in liver showed significantly increasing trend when DCL increased from 0% to 15%, followed by significant decline when DCL exceeds 15% (P < 0.05). The malondialdehyde (MDA) contents in liver were not affected by DCL. Based on these results, the optimal level of dietary carbohydrate supplementation was estimated at approximately 11% for maximum growth and normal metabolism of hybrid snakehead.

• 单位
  广东省农业科学院