A novel fluorescent probe for the detection of AmpC beta-lactamase and the application in screening beta-lactamase inhibitors

作者：Yu, Pan; Yang, Jia-Ning; Yan, Jin-Wu; Meng, Zhi-Zhong; Hong, W. David; Roberts, Adamp.; Ward, Stephen A.; Zhang, Lei^*; Li, Shan^*

来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy , 2020, 234.

DOI：10.1016/J.saa.2020.118257

• (oLactamase Inhibitor screening
• Fluorescence probe
• AmpC

The rapid detection of iii-lactamases (Blas) and effective screening of Bla inhibitors are critically important and urgent fur solving antibiotic resistance and improving precision medicine. Here a novel fluorescent probe CDC 559 was designed and synthesized, which can be used for the selective and direct detection of AmpC Blas. More importantly, it can realize screening the Bla inhibitors with sulbaclam sodium and tazobactam as model compounds, and the half-maximal inhibitory concentration are 0279 tiM and 0.053 tiM, respectively. CDC -559 can be applied not only to examine the resistance of bacterial strains, but also W categorize its mode of action specifically, which is consistent with the essential result of the Blas. The research suggests that CDC-559 probe has tremendous potential in the rapid detection of AmpC Blas as well as the strains with AmpC-encocled gene, which is instructive in promoting better antibiotic stewardship practices and developments. 2020 Elsevier B.V.

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