Recyclable laccase by coprecipitation with aciduric Cu-based MOFs for bisphenol A degradation in an aqueous environment

作者：Yuan, Yuhang; Cai, Wenting; Xu, Jiaxin; Cheng, Jianhua^*; Du, Ke-Si^*

来源：Colloids and Surfaces B: Biointerfaces , 2021, 204: 111792.

DOI：10.1016/j.colsurfb.2021.111792

• Copper-based metal-organic framework
• Immobilized laccase
• In-situ
• Bisphenol A removal

Copper-based MOF (Cu-PABA) was selected to immobilize laccase (Lac) at optimum pH because of its favorable acid resistance. Cu-PABA@Lac biocomposites were synthesized in situ by the one-step method under moderate conditions (water environment and normal temperature and pressure). Cu-PABA@Lac had great potential to maintain stability due to the protection of the Cu-PABA shell and reasonable conformational changes. In addition, Cu-PABA@Lac could be used repeatedly by centrifugation, as confirmed in the degradation experiment of bisphenol A (BPA). Because of the synergistic effect of copper ions between laccase and Cu-PABA, the Km value decreased (from 0.0024 to 0.0014 mM); therefore, the affinity between laccase and guaiacol was enhanced. In conclusion, the system provides a choice for immobilized acid-resistant enzymes and a solution for environmental BPA degradation.