A label-free ultrasensitive microRNA-21 electrochemical biosensor based on MXene (Ti3C2)-reduced graphene oxide-Au nanocomposites

作者：Guo, Shuzhou; Lin, Yaping; Lian, Ziqi; Zeng, Tao; Wang, Liangliang; Ye, Rongkai; Hong, Benlin; Liang, Qianwei^*; Wu, Qianqing; Hu, Jianqiang^*; Li, Aiqing^*

来源：Microchemical Journal, 2023, 190: 108656.

DOI：10.1016/j.microc.2023.108656

• MicroRNA
• Electrochemical biosensor
• MXene
• Gold nanoparticles
• Label-free

The detection of microRNA is widely used in the early diagnosis of diseases such as cancer and acute kidney injury. In this work, we successfully constructed a label-free ultrasensitive biosensor for the instant detection of miRNA-21. The MXene-reduced graphene oxide (rGO)-Au was synthesized as electrode materials to immobilize DNA probes and MXene-AuPd was used as signal amplification materials. The highly sensitive biosensor had a wide range of linearity from 1 fM to 1 nM, and the limit of detection of as-fabricated biosensor was lower as 0.42 fM under optimized conditions. The biosensor also showed high specificity, outstanding stability and desirable reproducibility, which could also realize the accurate detection of miRNA-21 in real urine samples of both mouse and human with high sensitivity. The label-free electrochemical biosensor based on MXene nanocomposites is a promising candidate for potential applications on the early diagnosis of cancers and acute kidney injury.

• 单位
  广州中医药大学; 南方医科大学; 1