Evaluation of the Effects of Anti-PD-1 Therapy on Triple-Negative Breast Cancer in Mice by Diffusion Kurtosis Imaging and Dynamic Contrast-Enhanced Imaging

作者：Tang, Wen-Jie; Yao, Wang; Jin, Zhe; Kong, Qing-Cong; Hu, Wen-Ke; Liang, Yun-Shi; Chen, Lei-Xin; Chen, Si-Yi; Zhang, Qiong-Qiong; Wei, Xin-Hua; Xu, Xiang-Dong^*; Guo, Yuan^*; Jiang, Xin-Qing^*

来源：Journal of Magnetic Resonance Imaging, 2022, 56(6): 1912-1923.

DOI：10.1002/jmri.28215

• immunotherapy
• triple-negative breast cancer
• diffusion kurtosis imaging
• dynamic contrast-enhanced imaging

Background The monitoring of immunotherapies is still based on changes in the tumor size in imaging, with a long evaluation period and low sensitivity. Purpose To investigate the effectiveness of diffusion kurtosis imaging (DKI) and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in assessing the therapeutic efficacy of anti-programmed death-1 (PD-1) therapy in a mouse triple negative breast cancer (TNBC) model. Study Type Prospective. Animal Model A total of 54 BALB/c mouse subcutaneous 4 T1 transplantation models of TNBC. Field Strength/Sequence A 3.0-T; turbo spin echo (TSE) T2-weighted imaging, DKI with seven b values (0, 500, 1000, 1500, 2000, 2500, and 3000 sec/mm(2)) and T1-twist DCE acquisition series. Assessment DKI and DCE-MRI parameters were evaluated by two radiologists independently. Regions of interest (ROIs) were drawn manually on the maximum cross-sectional area of the lesion; care was taken to avoid necrotic areas. The tumor cell density, the CD45 and CD31 levels were analyzed by two pathologists. Statistical Tests The two-tailed unpaired t-test, Mann-Whitney U test, Fisher's exact test and Pearson correlation coefficient were performed. A P < 0.05 was considered statistically significant. Results The apparent diffusion coefficient (ADC), mean diffusivity (MD), K-trans and K-ep values were significantly different between the two groups at each time point after treatment. There were significant differences in the mean kurtosis (MK) and V-e values between the two groups at 5 and 10 days after treatment but no significant differences at 15 days (P = 0.317 and 0.183, respectively). The ADC and MD values were significantly correlated with tumor cell density (ADC, r = -0.833; MD, r = 0.890) and the CD45 level (ADC, r = 0.720; MD, r = 0.718). The K-trans and K-ep values were significantly correlated with the CD31 level (K-trans, r = 0.820; K-ep, r = 0.683). Data Conclusion DKI and DCE-MRI could reflect the changes in tumor microstructure and tumor tissue vasculature after anti-PD-1 therapy, respectively. Level of Evidence 1 Technical Efficacy Stage 4.

• 单位
  1; 中山大学; y