Long-term performance and microbial ecology of a two-stage PN-ANAMMOX process treating mature landfill leachate

作者:Li Huosheng; Zhou Shaoqi*; Ma Weihao; Huang Pengfei; Huang Guotao; Qin Yujie; Xu Bin; Ouyang Hai
来源:Bioresource Technology, 2014, 159: 404-411.
DOI:10.1016/j.biortech.2014.02.054

摘要

Long-term performance of a two-stage partial nitritation (PN)-anaerobic ammonium oxidation (ANAMMOX) process treating mature landfill leachate was investigated. Stable partial nitritation performance was achieved in a sequencing batch reactor (SBR) using endpoint pH control, providing an effluent with a ratio of NO2--N/NH4+-N at 1.23 +/- 0.23. High rate nitrogen removal over 4 kg N/m(3)/d was observed in the ANAMMOX reactor in the first three months. However, during long-term operation, the ANAMMOX reactor can only stably operate under nitrogen load of 1 kg N/m(3)/d, with 85 +/- 1% of nitrogen removal. The ammonium oxidizing bacteria (AOB) in the PN-SBR were mainly affiliated to Nitrosomonas sp. IWT514, Nitrosomonas eutropha and Nitrosomonas eutropha, the anaerobic ammonium oxidizing bacteria (AnAOB) in the ANAMMOX reactor were mainly affiliated to Kuenenia stuttgartiensis.