Colorimetric assay of L-cysteine based on peroxidase-mimicking DNA-Ag/Pt nanoclusters

作者：Wu Liang Liang; Wang Li Ying; Xie Zheng Jun; Pan Na; Peng Chi Fang^*

来源：Sensors and Actuators, B: Chemical , 2016, 235: 110-116.

DOI：10.1016/j.snb.2016.05.069

• Ag/Pt nanoclusters
• Enzyme mimic
• L-Cysteine
• Colorimetric detection

This paper reported that the peroxidase-like activity of DNA-Ag/Pt nanoclusters (NCs) can be inhibited significantly by L-cysteine. Based on this finding, a highly sensitive and selective colorimetric detection for L-cysteine was developed with a detection limit (LOD) of 2.0 nM and a linear range from 5.0 nM to 500 nM. This detection for L-cysteine was highly selectivity over other amino acids. The high sensitivity and selectivity of this method facilitated the detection of L-cysteine in biological samples. Moreover, this method developed here was low-cost and simple. These results indicated the excellent analytical application of Ag/Pt NCs as enzyme mimic.

• 单位
  江南大学