The expression of two collagen receptor subfamilies, integrins and discoidin domains during osteogenic and chondrogenic differentiation of human mesenehymal stem cells

作者：Xue, Fan; Zhou, Wei; Lan, Zedong^*

来源：BIO-MEDICAL MATERIALS AND ENGINEERING, 2021, 32(4): 195-205.

DOI：10.3233/BME-201151

• Collagen receptors
• human mesenehymal stem cell
• osteogenesis
• chondrogenesis

BACKGROUND: Collagen receptors are characterized by binding to and being activated by collagens. We know little about the molecular mechanism by which the integrins and discoidin domains (DDRs) recognize collagen. @@@ OBJECTIVE: The aim of this study was to investigate the expression of two main collagen receptor subfamilies, integrins and DDRs, during osteogenic and chondrogenic differentiation of human mesenehymal stem cells (hMSCs). @@@ METHODS: Using qRT-PCR, Western blots and FACS, the levels of DDR1, DDR2, integrin subunits beta 1, alpha 1, alpha 2, alpha 10 and alpha 11 receptors on hMSCs, were assessed upon activation by collagen type I, as well as during osteogenic and chondrogenic differentiation. @@@ RESULTS: The expression of DDR2 and integrin alpha 11 beta 1 was altered compared with other receptors when the cells were cultured under undifferentiated conditions. During osteogenic and chondrogenetic differentiation, DDR2 and alpha 11 were upregulated during early stages (6 day) of osteogenesis and chondrogenesis, respectively. The expression and activation of DDR2 was concomitant with another receptor integrin subunit beta 1 during osteogenetic differentiation. @@@ CONCLUSIONS: The results suggested that DDR2 was more specific for osteogenesis than chondrogenesis, while integrin alpha 11 beta 1 was more specific in chondrogenesis. DDR2 and alpha 11 may play a role in the regulation of osteogenesis and chondrogenesis based on the differential expression of these receptors during lineage-dependent changes.

• 单位
  南方医科大学