Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

作者：Yih Yih KOK; Wan Loy CHU; Siew Moi PHANG; Shar Mariam MOHAMED; Rakesh NAIDU; Pey Jiun LAI; Shui Nyuk LING; Joon Wah MAK; Patricia Kim Chooi LIM; Pauline BALRAJ; Alan Soo Beng KHOO

来源：Journal of Zhejiang University-Science B(Biomedicine & Biotechnology), 2011, 335-345.

• Microalgae
• Ankistrodesmus convolutus
• Synechococcus elongatus
• Spirulina platensis
• Lymphoblastoid cells
• Epstein-Barr virus(EBV)

This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus,Synechococcus elongatus,and Spirulina platensis against Epstein-Barr virus(EBV) in three Burkitt’s lymphoma(BL) cell lines,namely Akata,B95-8,and P3HR-1.The antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction(PCR) technique.The methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in re-ducing cell-free EBV DNA(EC50<0.01 μg/ml) with a high therapeutic index(>28 000).After fractionation by column chromatography,the fraction from Synechococcus elongatus(SEF1) reduced the cell-free EBV DNA most effectively(EC50=2.9 μg/ml,therapeutic index>69).Upon further fractionation by high performance liquid chromatography(HPLC),the sub-fraction SEF1’a was most active in reducing the cell-free EBV DNA(EC50=1.38 μg/ml,therapeutic index>14.5).This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.

• 单位
  University of Malaya