Light-controlled and glutathione-stimulative DNA walking motor under the collaborative carrying of MOF for self-powered sensing and drug release in living organisms

摘要

Although the DNA walking motor is a good biosensing means for fluorescence imaging in living organisms, the unsatisfying sensing precision, demand of exogenous powering forces and exploration of collaborative carriers bring a difficulty to integrate diagnosis with therapy. To address these challenges, some resultful solutions are proposed here. First, a photocleavage-bond is incorporated into the nucleic acid modules to actualize a lightcontrolled action, by which a simple external illumination of a 365 nm ultraviolet light can spatiotemporally expose the target identification domain to prevent the sensing response from being pre-initiated during biological delivery. After this, a MnO2 nanosheet endowed glutathione-stimulative concept is used to produce abundant self-supplied cofactors for a Mn2+-dependent DNAzyme, under which a three-dimensional self-powered DNA walking phenomenon is executed on the surface of a metal-organic framework (UiO-66) collectively carried with the sensing system and a chemical drug (doxorubicin). By electing an underlying wide-spectrum cancer biomarker (survivin messenger RNA) as a conceptual demonstration, our highly integrated DNA walking motor exhibits not only favorable in vitro sensing performance but also a high-efficiency synergistic drug release manner. Moreover, robust imaging capability and chemotherapy course are proved in living cells and mouse bodies, showing a favorable application potential.

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