Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge

作者：Jiang, Junfeng; Zeng, Tao; Zhang, Li; Fan, Xingfei; Jin, Qishu; Ni, Haitao; Ye, Yusheng; Cheng, Lipeng; Li, Li; Wang, Liujun; Xu, Sha; Yang, Yu; Gu, Juan; Guo, Bing; Wang, Lei; Li, Xin; Qin, Yingyi; Li, Jiaxi; Wang, Jinjiang; Chen, Xi; Wu, Minjuan; Ying, Qi-long^*; Qin, Xingjun^*; Wang, Yefei^*; Wang, Yue

来源：Molecular Cancer, 2022, 21(1): 136.

DOI：10.1186/s12943-022-01604-x

• CRISPR-Cas9
• miRNA sponge
• Let-7
• RNA sequence optimization

HighlightsCas9 RNA functions as a miRNA sponge.Let-7 is the dominant regulated miRNA by Cas9 RNA.RNA sequence optimization of Cas9 by synonymous mutation improves its safety.

• 单位
  上海交通大学; 哈尔滨医科大学; y