MicroRNA-2355-5p regulates γ-globin expression in human erythroid cells by inhibiting KLF6

作者：Cheng, Yi; Shang, Xuan; Chen, Diyu; Pang, Dejian; Zhao, Cunyou; Xu, Xiangmin^*

来源：British Journal of Haematology, 2021, 193(2): 401-405.

DOI：10.1111/bjh.17134

• KLF6
• &#947
• &#8208
• globin
• miR&#8208
• 2355&#8208
• 5p
• HbF
• &#946
• thalassaemia

Kruppel-like factors (KLFs) are a highly conserved family of transcription factors. We analysed expression profile data of KLFs and identified KLF6 as a new potential regulator of erythropoiesis. Knocking down the expression of KLF6 significantly raised gamma-globin mRNA and protein levels in the erythroid cell line HUDEP-2 and haematopoietic progenitor (CD34(+)) cells. We found that overexpression of microRNA (miR)-2355-5p in HUDEP-2 and CD34(+) cells correlated with increased gamma-globin synthesis by suppressing expression of KLF6. Our discovery that the interaction between miR-2355-5p and KLF6 affects the expression of gamma-globin may provide more information for the clinical management of beta-thalassaemia patients.

• 单位
  南方医科大学