MicroRNA-2355-5p regulates γ-globin expression in human erythroid cells by inhibiting KLF6

摘要

Kruppel-like factors (KLFs) are a highly conserved family of transcription factors. We analysed expression profile data of KLFs and identified KLF6 as a new potential regulator of erythropoiesis. Knocking down the expression of KLF6 significantly raised gamma-globin mRNA and protein levels in the erythroid cell line HUDEP-2 and haematopoietic progenitor (CD34(+)) cells. We found that overexpression of microRNA (miR)-2355-5p in HUDEP-2 and CD34(+) cells correlated with increased gamma-globin synthesis by suppressing expression of KLF6. Our discovery that the interaction between miR-2355-5p and KLF6 affects the expression of gamma-globin may provide more information for the clinical management of beta-thalassaemia patients.

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