Walnut-derived peptide PPKNW alleviate polystyrene microparticles-induced growth inhibition of Lactobacillus rhamnosus GG

作者：Xu, Yongzhao; Nie, Shiying; Wang, Min; Zhao, Zikuan; Amakye, William Kwame; Yuan, Erdong; Ren, Jiaoyan^*

来源：Food Bioscience, 2023, 53: 102528.

DOI：10.1016/j.fbio.2023.102528

• Polypeptide
• Microplastic
• PW5
• Lactobacillus rhmnosus GG
• Probiotics
• Interaction

Microplastic (MP) is ubiquitous and makes human exposure inevitable through the consumption of food. The impact of MP on the gut microbiome and human health is still unclear. Here, we investigated the ability of food derived peptides to counteract the inhibitory effect of polystyrene microplastic (PS-MP) on probiotics and elucidated the underlying mechanism. Pro-Pro-Asn-Lys-Trp (PW5,100 mu g/mL), derived from walnut-protein, significantly reversed the growth inhibition of Lactobacillus rhamnosus by PS500 (Area under the curve increased by 13.74%, p < 0.01). Furthermore, the effectiveness of orally administered PW5 was explored by NMR, our results suggest that the intact peptide is necessary for the observed activity of PW5.PW5 and PS500 primarily interact through hydrogen bonding and van der Waals forces to form new complexes. Together, our data demonstrated the potential mechanism by which food-derived peptides bind to PS-MP to reduce the inhibitory effect of MP on probiotic growth, and opens a new perspective for the application of food-derived bioactive peptides in reducing the growing health risks posed by MP.