Quantitative Analysis of Stall of Replicating DNA Polymerase by G-Quadruplex Formation.

Authors：Takahashi, Shuntaro; Sugimoto, Naoki^*

Source：Methods Mol Biol, 2019, 2035: 257-274.

DOI：10.1007/978-1-4939-9666-7_15

• Circular dichroism
• Gel electrophoresis
• Kinetic analysis
• Molecular crowding
• Replication
• Thermodynamics

The formation of guanine-quadruplexes (G4s) in genomic DNA and RNA inhibits replication, transcription, and translation. Although several regions of the human genome exhibit G4-forming potential, the stability and topology of the G4s formed vary depending on the molecular environment. Here, we describe a protocol to quantitatively analyze the inhibitory effects of G4s with different stabilities and topologies on replication in conditions of molecular crowding.