Identification of phytochemicals in Qingfei Paidu decoction for the treatment of coronavirus disease 2019 by targeting the virus-host interactome

作者：Li, Yuyun; Wu, Yan; Li, Siyan; Li, Yibin; Zhang, Xin; Shou, Zeren; Gu, Shuyin; Zhou, Chenliang; Xu, Daohua; Zhao, Kangni; Tan, Suiyi; Qiu, Jiayin^*; Pan, Xiaoyan^*; Li, Lin^*

来源：Biomedicine and Pharmacotherapy, 2022, 156: 113946.

DOI：10.1016/j.biopha.2022.113946

• COVID-19
• Qingfei Paidu Decoction
• SARS-CoV-2
• Bioinformatics
• Systematic pharmacology
• Virus -host interactome

Qingfei Paidu decoction (QFPDD) has been clinically proven to be effective in the treatment of coronavirus disease 2019 (COVID-19). However, the bioactive components and therapeutic mechanisms remain unclear. This study aimed to explore the effective components and underlying mechanisms of QFPDD in the treatment of COVID-19 by targeting the virus-host interactome and verifying the antiviral activities of its active components in vitro. Key active components and targets were identified by analysing the topological features of a compoundtarget-pathway-disease regulatory network of QFPDD for the treatment of COVID-19. The antiviral activity of the active components was determined by a live virus infection assay, and possible mechanisms were analysed by pseudotyped virus infection and molecular docking assays. The inhibitory effects of the components tested on the virus-induced release of IL-6, IL-18 and CXCL-10 were detected by ELISA. Three components of QFPDD, oroxylin A, hesperetin and scutellarin, exhibited potent antiviral activities against live SARS-CoV-2 virus and HCoV-OC43 virus with IC50 values ranging from 18.68 to 63.27 mu M. Oroxylin A inhibited the entry of SARS-CoV-2 pseudovirus into target cells and inhibited SARS-CoV-2 S protein-mediated cell-cell fusion by binding with the ACE2 receptor. The active components of QFPDD obviously inhibited the IL-6, IL-18 and CXCL-10 release induced by the SARS-CoV-2 S protein. This study supports the clinical application of QFPDD and provides an effective analysis method for the in-depth study of the mechanisms of traditional Chinese medicine (TCM) in the prevention and treatment of COVID-19.

• 单位
  广东医学院; 广州中医药大学; 南方医科大学