The therapeutic effect of IL-21 combined with IFN-γ inducing CD4+CXCR5+CD57+T cells differentiation on hepatocellular carcinoma

作者：Zhao, Changlin^*; Wu, Xianlin; Chen, Jia; Qian, Guoqiang

来源：Journal of Advanced Research, 2022, 36: 89-99.

DOI：10.1016/j.jare.2021.05.010

• Hepatocellular carcinoma
• IL-21
• IFN-gamma
• CD4(+)CXCR5(+)CD57(+)T cells
• Treg
• Tumor microenvironment

Introduction: Liver cancer is a malignant tumor with high incidence and short survival time. In order to increase the cure rate and disease-free survival rate of liver cancer, it is necessary to seek effective treatment methods. @@@ Objectives: The objective of this study is to evaluate the therapeutic effects of IL-21 and IFN-gamma inducing the formation of CD4(+)CXCR5(+)CD57(+)T cells on liver cancer. @@@ Methods: The methods of analyze the relationship between CD4(+)CXCR5(+)CD57(+)T cells and the survival time of hepatocellular carcinoma (HCC), and study the effect of IL-21 combined with IFN-gamma in inducing stem cells to differentiate into CD4(+)CXCR5(+)CD57(+)T cells. The effects of IL-21 combined with IFN-gamma induced CD4(+)CXCR5(+)CD57(+)T cells on liver cancer were studied through animal experiments, and the regulatory mechanism, and the effect of hepatitis B virus (HBV) on it. @@@ Results: The study found that the number of CD4(+)CXCR5(+)CD57(+)T cells in serum of liver cancer patients with prolonged survival time increased significantly, the expression of CD4, CD57, and CXCR5 in the tumor microenvironment increased, and the serum IL-21 and IFN-gamma concentrations increased. IL-21 and IFN-gamma induce stem cells to differentiate into CD4(+)CXCR5(+)CD57(+)T cells and induce HepG2 cells apoptosis. HBV leads to a decrease in the number of CD4(+)CXCR5(+)CD57(+)T cells and a chronic inflammatory response. Treg cells can regulate CD4(+)CXCR5(+)CD57(+)T cells. IL-21 combined with IFN-gamma induced an increase in the number of CD4(+)CXCR5(+)CD57(+)T cells in hepatocarcinoma-bearing mice, which has an inhibitory effect on H22 liver cancer. @@@ Conclusion: The conclusion of the study is that IL-21 combined with IFN-gamma induces stem cells to differentiate into CD4(+)CXCR5(+)CD57(+)T cells, Treg can control the increase in their number, and HBV can cause their number to decrease, which can control the growth of liver cancer.

• 单位
  1; 广东药学院