Study on the Antioxidant Effects of Suaeda rigida Polysaccharides In Vitro and In Vivo

摘要

The objectives of this study were to investigate the antioxidant effects of Suaeda rigida polysaccharide (SRPs) in vitro and in vivo. Three free radical scavenging experiments, including DPPH, center dot OH and O-2(center dot-) were used to study the antioxidant effects of the SRPs in vitro. The antioxidant effects of three concentrations of the SRPs on the immunosuppressive mice were analyzed by UV-Vis spectrophotometry and chemical colorimetry. The study is mainly from the aspects of the NOS-NO-cGMP signaling pathway, enzymatic and non-enzymatic antioxidant systems, and immune organ index. The results showed that the SRPs could effectively scavenge the DPPH, center dot OH and O-2(center dot-) in vitro, and the scavenging rate increased with the increase of its concentration in a specific range. In addition, the SRPs could significantly affect the NOS-NO-cGMP signaling pathway, enzymatic and non-enzymatic antioxidant systems (CAT, GSH-Px, GST, GSH, etc.), and immune organ index in the immunosuppressive mice, so as to exert the antioxidant effects. All the results suggested that the SRPs had significant antioxidant effects in vitro and in vivo. The mechanisms were related to scavenging the DPPH, center dot OH and O-2(center dot-) in vitro, reducing the contents of center dot OH, MDA, H2O2, increasing the GSH content, enhancing the activities of CAT, GSH-Px, GST, and weakening the XOD activity, finally inhibiting the NOS-NO-cGMP signaling pathway in the immunosuppressed mice.

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