Chemiluminescence of lucigenin-tetracycline and its application for sensitive determination of procyanidin

作者：Quan, Shuai; Ji, Kaixiang; Liu, Fangshuo; Barkae, Tesfaye Hailemariam; Halawa, Mohamed Ibrahim; Hanif, Saima; Lou, Baohua^*; Li, Jianping^*; Xu, Guobao^*

来源：JOURNAL OF FOOD AND DRUG ANALYSIS, 2022, 30(2): 293-302.

DOI：10.38212/2224-6614.3411

• Chemiluminescence
• Lucigenin
• Procyanidin
• Tetracycline

Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10-1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.

• 单位
  桂林理工大学; 中国科学院