Anti -oxidant, anti -bacterial and anti-biofilm activity of biosynthesized silver nanoparticles using Gracilaria corticata against biofilm producing K. pneumoniae

作者：Rajivgandhi, Govindan Nadar; Ramachandran, Govindan; Maruthupandy, Muthuchamy; Manoharan, Natesan; Alharbi, Naiyf S.; Kadaikunnan, Shine; Khaled, Jamal M.; Almanaa, Taghreed N.; Li, Wen-Jun

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2020, 600.

• Marine seaweed
• Silver nanoparticle
• Antioxidant activity
• Minimum inhibition concentration
• Biofilm metabolic activity
• Toxicity effect

Silver nanoparticles (Ag NPs) with anti -bacterial effects against the biofilm-producing bacteria Klebsiella pneu- moniae ( K. pneumoniae ) were synthesized using the marine seaweed Gracilaria corticata ( G. corticata ). Physiochemical characterization using UV -spectrometer, fourier transform infrared spectroscopy (FTIR) and X- ray diffraction (XRD) confirmed that the synthesized material consisted of Ag NPs. Morphological analysis using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) with energy -dispersive X-ray (EDX) analysis confirmed the shape, size, surface morphology, and available chemical composition of the Ag NPs. Furthermore, the rich phenolic and flavonoid content of G. corticata has excellent antioxidant activity, which was confirmed through gas chromatography -mass spectrometry (GC -MS) analysis. The highest anti- bacterial activity and biofilm reduction (88 %) of Ag NPs treated K. pneumoniae was observed at 50 ?g/mL and 100 ?g/mL concentrations respectively. The bacterial viability and exopolysaccharide production of K. pneu- moniae significantly decreased after treatment with Ag NPs. Morphological alterations and intracellular damage

• 单位
  中山大学; 中国科学院新疆生态与地理研究所