Red-emitting rhodamine-based probe with large Stokes shift for ClO-detection

Authors：Liang, Qin; Chen, Wenqiang; Ren, Xiaojie^*; Song, Xiangzhi^*

Source：Tetrahedron, 2022, 127: 133020.

DOI：10.1016/j.tet.2022.133020

• Rhodamine
• Dyes
• Probe
• Hypochlorite

A fluorescent rhodamine dye, HN, was prepared by introducing tetrahydroquinoxalinyl donor, which displayed far-red emission (687 nm), large Stokes shift (84 nm), and relatively high fluorescent quantum yield (0.33). Based on HN dye, a fluorescent probe, HN-HClO, was developed for hypochlorite (ClO-) detection. The probe HN-HClO exhibited fast response time (within 100 s), excellent sensitivity (LOD = 74 nM), and good selectivity toward ClO-. The probe HN-HClO was capable of imaging ClO- in both living cells and zebrafish. It could also monitor the endogenously generated ClO- in the MPO-catalyzed system.