Interaction Dynamics Between Dacarbazine and DNA

作者：WANG Huan; WANG Jiao; LI Zong xiao; ZHAO Wei wei; PU Xiao hua; CHENG Hua lei

来源：发光学报, 2016, 37(12).

DOI：10.3788/fgxb20163712.1560

• DNA
• dacarbazine
• spectroscopy
• interaction mechanism
• isothermal titration calorimetry

The interaction between calf thymus DNA and anti-cancer drug dacarbazine was investiga-ted using isothermal titration calorimetry( ITC) , spectroscopy, viscosity and other methods. The hy-perchromic effect and blue shift in the absorption spectra as well as viscosity decline are observed clearly after the interaction between dacarbazine and DNA. Furthermore, the binding constant and number of binding sites are also recorded by ITC, presenting that the interactions of the dacarbazine with DNA can be categorized for two modes: a non-classical intercalation type and surface binding effect. In former,△H1 <0, △S1 > 0, K1 =5. 63 × 104 , the binding site is 0. 10. In latter, only surficial interaction during the combination of the drug molecule with DNA occurs, not embedding into the hydrophobic part of the DNA molecule, due to△H2 > 0 ,△S2 > 0, K2 =1. 00 × 103 , and the binding site of 9. 99. Meanwhile, UV method is also employed to find the binding constant of Ka=6. 70 × 104 , which is consistence with ITC observation.

• 单位
  宝鸡文理学院; 化学化工学院