Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents

作者：Xiong, Jun; Cao, Shi-Lin; Zong, Min-Hua; Lou, Wen-Yong^*; Wu, Xiao-ling^*

来源：Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 2020, 192(2): 573-584.

DOI：10.1007/s12010-020-03345-3

• Papain
• Nanocrystalline cellulose
• Alanyl-histidine
• Dipeptide synthesis
• Deep eutectic solvents

Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide. The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%). Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces. In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.

• 单位
  佛山科学技术学院