Guided isolation of secondary metabolites from Nectria sp. MHHJ-3 by molecular network strategy

作者：Zhu, Li; Gan, Dong; Dong, Shu-Hui; He, Bi-Jian; Li, Chen-Zhe; Wang, Cheng-Yao; Cai, Lan; Su, Jin-Wei; Cai, Le; Ding, Zhong-Tao^*

来源：FITOTERAPIA, 2023, 171: 105668.

DOI：10.1016/j.fitote.2023.105668

• Nectria sp.
• Molecular networking
• Secondary metabolite
• Bioactivity

The fungus Nectria sp. MHHJ-3 was isolated from Illigera rhodantha. A molecular networking-guided the secondary metabolites investigation of Nectria sp. MHHJ-3 led to the isolation of ten metabolites (1-10), including two new naphthalenone derivatives, nectrianaphthalenones A (1) and B (2), and two new steroids, nectriasteroids A (3) and B (4). Their structures were elucidated by extensive spectroscopic analysis including the HRESIMS, 1D/2D NMR and electronic circular dichroism (ECD) spectra. A plausible biosynthetic pathway for 1-2 was proposed. Compounds 1 and 2 exhibited moderate acetylcholinesterase (AChE) inhibitory activities. Compounds 3 and 4 showed significant cytotoxic activity against selected tumor cells. Particularly, compound 3 exhibited the strongest activity against A549 cells with an IC50 value of 13.73 +/- 0.03 mu M, which was at the same grade with that of positive control cisplatin.

• 单位
  云南大学