Identification and Structure-Activity Relationship of Recovered Phenolics with Antioxidant and Antihyperglycemic Potential from Sugarcane Molasses Vinasse

作者：Huang, Zhe; Chen, Yinning; Huang, Riming; Zhao, Zhengang^*

来源：Foods, 2022, 11(19): 3131.

DOI：10.3390/foods11193131

• molasses vinasse
• phenolic acids
• recycling
• antioxidant
• antihyperglycemic
• structure-activity relationship

Sugarcane molasses vinasse is the residue of the fermentation of molasses and the water and soil environmental pollutants from distilleries. However, its recycling value has been neglected. The chemical analysis of the molasses vinasse led to the isolation of a new benzoyl chloride called 2,3,4-trihydroxy-5-methoxy benzoyl chloride, as well as thirteen known compounds, including six benzoic acids. The structure of the new benzoyl chloride was elucidated on the basis of extensive spectroscopic analysis. The antioxidant activity of all isolated compounds was measured using the ORAC assay. Moreover, we compared the cellular antioxidant activity (CAA) and inhibitory activity against alpha-amylase and alpha-glucosidase for structure-activity analysis. The results showed that only vanillic acid had CAA (8.64 mu mol QE/100 mu mol in the no PBS wash protocol and 6.18 mu mol QE/100 mu mol in the PBS wash protocol), although other benzoic acid derivatives had high ORAC values ranging between 1879.9 and 32,648.1 mu mol TE/g. Additional methoxy groups at the ortho-positions of the p-hydroxy group of benzoic acids enhanced the inhibition of alpha-glucosidase but reduced the ORAC activity unless at the para-position. This work indicated that phenolics, especially phenolic acids in the sugarcane molasses vinasse, possessed potential antioxidant and antihyperglycemic activity, which improved the utilization rate of resources and reduced the discharge of pollutants.

• 单位
  广西大学; 华南农业大学