Identification of differentially expressed miRNAs and key genes involved in the progression of alcoholic fatty liver disease using rat models

Authors：Zhang, Xuemei; Song, Wenqi; Zhang, Mingxuan; Song, Yuanhang; Di, Yuzhu; Chen, Bo; Tian, Hao; Yuan, Xixian; Jin, Shizhu^*

Source：CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY, 2022, 46(10): 102012.

DOI：10.1016/j.clinre.2022.102012

• AFLD
• Bioinformatics analysis
• Sequencing
• Gene expression omnibus (GEO)

Background: Alcoholic fatty liver disease (AFLD) is a liver disease caused by prolonged heavy drinking and has a poor prognosis in the clinic. This study aimed to explore the differential miRNAs expression profiles in the AFLD rat model. Methods: The rat model of AFLD was established by ethanol intragastric administration and was used to explore the differential miRNAs expression profiles. We further analyzed the potential target mRNAs using the bioinformatics technique. GO and KEGG pathway enrichment analyses were carried out to better understand the biological function of differential expression genes (DEGs). We used the human Gene Expression Omnibus (GEO) dataset GSE28619 to further screen the key differentially expressed genes. The integration between the differentially expressed genes from the AFLD model and GEO was conducted and the key genes were identified.

• Institution
  1; 哈尔滨医科大学