MicroRNA-107 Inhibits U87 Glioma Stem Cells Growth and Invasion

作者：Chen Lei; Chen Xiang rong; Chen Fan fan; Liu Yi; Li Peng; Zhang Run; Yan Ke; Yi Yong jun; Xu Zhi min; Jiang Xiao Dan^*

来源：Cellular and Molecular Neurobiology, 2013, 33(5): 651-657.

DOI：10.1007/s10571-013-9927-6

• miR-107
• Glioma stem cells
• Proliferation
• Invasiveness
• Notch2

Glioma stem cells (GSCs) are thought to be critical for resistance to radiotherapy and chemotherapy and for tumor recurrence after surgery in glioma patients. Identification of new therapeutic strategies that can target GSCs may thus be critical for improving patient survival. MicroRNAs (miRNAs) are small non-coding RNAs that function as tumor suppressors or oncogenes. In this study, we confirmed that miR-107 was down-regulated in GSCs. To investigate the role of miR-107 in tumorigenesis of GSCs, a lentiviral vector over-expressing miR-107 in U87GSCs was constructed. We found that over-expression of miR-107 suppressed proliferation and down-regulated Notch2 protein and stem cell marker (CD133 and Nestin) expression in U87GSCs. Furthermore, enhanced miR-107 expression significantly inhibited U87GSC invasion and reduced matrix metalloproteinase-12 expression. miR-107 also suppressed U87GSCs xenograft growth in vivo. These findings suggest that miR-107 is involved in U87GSCs growth and invasion and may provide a potential therapeutic target for glioma treatment.

• 单位
  南方医科大学; 福建医科大学