Genotype identification and genetic diversity of Sugarcane yellow leaf virus in China

作者：Wang M Q^*; Xu D L; Li R; Zhou G H

来源：Plant Pathology, 2012, 61(5): 986-993.

DOI：10.1111/j.1365-3059.2012.02591.x

• genotypic diversity
• geographical distribution
• RT-PCR-RFLP
• Saccharum
• Sugarcane yellow leaf virus

A survey of Sugarcane yellow leaf virus (SCYLV) in China showed infection rates of 1.142.4% in six different sugarcane growing provinces, with an average rate of 20.3% (129/634), and no infection in one province. The majority of the infected plants showed yellowing of the midrib on the abaxial surface of leaves. Genotypic diversity of the 129 SCYLV isolates was analysed by a reverse transcriptionpolymerase chain reactionrestriction fragment length polymorphism (RT-PCR-RFLP) assay and nucleotide sequencing. Sixty-six isolates from Guangxi, Yunnan, Hainan, Fujian and Guizhou provinces were of the genotype BRA, whereas another 63 isolates from Guangdong province consisted of five genotypes, BRA, CHN1, CUB, PER and a novel genotype, CHN2. Among these genotypes, BRA was the predominant one identified in 43 samples (68.3%). Mixed infection of BRA and PER was also found in two samples. Phylogenetic analysis based on a 1.3-kb nucleotide sequence (including ORFs 34 and partial ORF 5) supported the identification of the novel CHN2 genotype. CHN2 shared 91.997.8% identity with five other known genotypes and was most closely related to the CHN1 genotype (97.697.8% identities). The nearly complete genomic sequences of seven isolates representing genotypes BRA, CUB and PER were determined, and they shared nucleotide sequence identities of 97.297.5%, 98.498.9% and 98.4%, respectively, with previously reported sequences of the corresponding genotypes.

• 单位
  华南农业大学; 云南省农业科学院